Advanced Photonics
22 Sep 2023
Fluorescence interference structured illumination microscopy for 3D morphology imaging with high axial resolution
Yile Sun1, Hongfei Zhu2, Lu Yin3, Hanmeng Wu1, Mingxuan Cai1, Weiyun Sun4, Yueshu Xu1,5, Xinxun Yang1, Jiaxiao Han1, Wenjie Liu1, Yubing Han1,6, Xiang Hao1, Renjie Zhou2, Cuifang Kuang1,5,7, Xu Liu1,5,7
1 Zhejiang University, College of Optical Science and Engineering, State Key Laboratory of Extreme Photonics and Instrumentation, Hangzhou, China
2 The Chinese University of Hong Kong, Department of Biomedical Engineering, Hong Kong, China
3 China Jiliang University, College of Optical and Electronic Technology, Hangzhou, China
4 Zhejiang University of Technology, Institute of Pharmacology, College of Pharmaceutical Sciences, Hangzhou, China
5 ZJU-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou, China
6 Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics-MoE Key Laboratory for Biomedical Photonics, Advanced Biomedical Imaging Facility-Wuhan National Laboratory for Optoelectronics, Wuhan, China
7 Shanxi University, Collaborative Innovation Center of Extreme Optics, Taiyuan, China
† These authors contributed equally to this work.
10.1117/1.AP.5.5.056007
We report a new imaging modality, fluorescence interference structured illumination microscopy (FI-SIM), which is based on three-dimensional structured illumination microscopy for wide-field lateral imaging and fluorescence interference for axial reconstruction. FI-SIM can acquire images quickly within the order of hundreds of milliseconds and exhibit even 30 nm axial resolution in half the wavelength depth range without z-axis scanning. Moreover, the relatively low laser power density relaxes the requirements for dyes and enables a wide range of applications for observing fixed and live subcellular structures.
Anhui Province Academy of Science and Technology, No. 128,Wanshui Road, Hi-Tech Zone, Hefei City, Anhui Province, China
+86 17354022649
service@sondii.com
IPv6 network supported
Our hours
Mon 11/21 - Wed 11/23: 9 AM - 8 PM
Thu 11/24: closed - Happy Thanksgiving!
Fri 11/25: 8 AM - 10 PM
Sat 11/26 - Sun 11/27: 10 AM - 9 PM
(all hours are Eastern Time)
