Journal of Biophotonics
02 August 2022
Background-free wide-field fluorescence imaging using edge detection combined with HiLo
Yuyao Hu1,2 ,Dong Liang1,3 ,Jing Wang4,5 ,Yaping Xuan1,2 ,Fu Zhao1,2 ,Jun Liu1,2* ,Ruxin Li1,2
1 State Key Laboratory of High Field Laser Physics and CAS Center for Excellence in Ultra-intense Laser Science, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai, China
2 University Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, China
3 School of Physics Science and Engineering, Tongji University, Shanghai, China
4 Institute of Photonic Chips, University of Shanghai for Science and Technology, Shanghai, China
5 Centre for Artificial-Intelligence Nanophotonics, School of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai, China
10.1002/jbio.202200031
A virtual HiLo based on edge detection (V-HiLo-ED) method is proposed for background removing of imaging. V-HiLo-ED is a novel method reconstructs the background-free image by estimating the foreground, which is different from all the existing popular software algorithms that estimate the background. V-HiLo-ED owns a better quality than state-of-the-art softwarebased methods and exhibits almost the same performance, but at faster speed compared with hardware-based methods.
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