Nano Research Journal Cover Design Service (Ag NWs)

Nano Research

07 October 2022

In-situ formation of “electron conductive wires” threaded ZIF-8 membrane for multiplexed immunoassay of human interleukins

Yu Lv¹, Mingshi Deng¹, Xuefeng Wang², Xinghua Gao¹, Lingyan Feng¹, Wei Chen^3✉, Chung-Chiun Liu⁴, Xinxin Li², Yuan Zhang^1✉

10.1007/s12274-022-5030-y

The immunodetection of ILs is based on the direct signal transduction ability of Ag NWs and specific interaction of periodically arranged Zn2+ ions in ZIF-8 films with biomolecules, which offer the high assay sensitivity and good specificity. The immunosensor chip achieves a wide detection range from pg/mL to ng/mL and possesses the ability to resist non-specific proteins adsorption in biological complex media. Further clinical serum samples assay verifies that the combination of IL-6 and IL-8 levels yields high diagnostic accuracy. Principal component analysis (PCA) reveals that 18 patient samples could be fully separated from healthy control samples. The low-cost, easily fabricated electrochemical immunosensing platform provides a rapid serum test for diagnosis and personalized therapy of inflammatory diseases, and can also be generalized to other immunoreaction-based biomarkers detections.