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  • Small

  • 28 October 2024

  • Bright and Stable Cyan Fluorescent RNA Enables Multicolor RNA Imaging in Live Escherichia coli

  • Li Jiang1,2,†, Fangting Zuo1,3,4,†, Yuanyuan Pan1,†, Ruilong Li1, Yajie Shi1, Xinyi Huang2, Dasheng Zhang1, Yingping Zhuang4, Yuzheng Zhao1,3,5, Qiuning Lin2, Yi Yang1,3, Linyong Zhu1,2,* and Xianjun Chen1,3,5,*

  • 1 Optogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237 China

    2 School of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai, 200240 China

    3 Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237 China

    4 School of Bioengineering, East China University of Science and Technology, Shanghai, 200237 China

    5 Research Unit of New Techniques for Live-cell Metabolic Imaging, Chinese Academy of Medical Sciences, Beijing, 100730 China

    † L.J., F.Z., and Y.P. contributed equally to this work.

  • 10.1002/smll.202405165

  • In article number 2405165, Linyong Zhu, Xianjun Chen, and co-workers designed a novel cyan fluorescent RNA (FR) called Myosotis with high cellular brightness and photostability. Myosotis is biorthogonal to Pepper and Clivia FRs, allowing multiplex fluorescence imaging of RNA in live bacteria.

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