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28 October 2024
Bright and Stable Cyan Fluorescent RNA Enables Multicolor RNA Imaging in Live Escherichia coli
Li Jiang1,2,†, Fangting Zuo1,3,4,†, Yuanyuan Pan1,†, Ruilong Li1, Yajie Shi1, Xinyi Huang2, Dasheng Zhang1, Yingping Zhuang4, Yuzheng Zhao1,3,5, Qiuning Lin2, Yi Yang1,3, Linyong Zhu1,2,* and Xianjun Chen1,3,5,*
1 Optogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237 China
2 School of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai, 200240 China
3 Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237 China
4 School of Bioengineering, East China University of Science and Technology, Shanghai, 200237 China
5 Research Unit of New Techniques for Live-cell Metabolic Imaging, Chinese Academy of Medical Sciences, Beijing, 100730 China
† L.J., F.Z., and Y.P. contributed equally to this work.
10.1002/smll.202405165
In article number 2405165, Linyong Zhu, Xianjun Chen, and co-workers designed a novel cyan fluorescent RNA (FR) called Myosotis with high cellular brightness and photostability. Myosotis is biorthogonal to Pepper and Clivia FRs, allowing multiplex fluorescence imaging of RNA in live bacteria.
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