Virologica Sinica
26 April 2024
The infection kinetics and transmission potential of two Guaico Culex viruses in Culex quinquefasciatus mosquitoes
Ru-Yi Chena,b,1, Teng Zhaob,1, Jing-Jing Guob,1, Feng Zhub,c,1, Na-Na Zhangb, Xiao-Feng Lib, Hai-Tao Liub, Fei Wanga,*, Yong-Qiang Dengb,*, Cheng-Feng Qinb,*
a College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, 330045, China
b State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, China
c School of Life Sciences, Southwest Forestry University, Kunming, 650224, China
1 Ru-Yi Chen, Teng Zhao, Jing-Jing Guo, and Feng Zhu contributed equally to this work and shared the first authorship.
10.1016/j.virs.2024.03.002
Guaico Culex virus (GCXV) is a newly identified segmented Jingmenvirus from Culex spp. mosquitoes in Central and South America. The genome of GCXV is composed of four or five single-stranded positive RNA segments. However, the infection kinetics and transmission capability of GCXV in mosquitoes remain unknown. In this study, we used reverse genetics to rescue two GCXVs (4S and 5S) that contained four and five RNA segments, respectively, in C6/36 cells. In conclusion, our results demonstrated that GCXVs with four or five RNA segments can be detected in Cx. quinquefasciatus eggs during the first and second gonotrophic cycles after oral infection.
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