Analytical Chemistry
19 July 2020
Tagging Transferrin Receptor with a Disulfide FRET Probe To Gauge the Redox State in Endosomal Compartments
Xiaobao Bi1,*,∥, Juan Yin2,∥, Dingpeng Zhang1, Xiaohong Zhang1, Seetharamsing Balamkundu3, Julien Lescar1, Peter C. Dedon3, James P. Tam1,* and Chuan-Fa Liu1,*
1 School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore
2 Program in Neuroscience and Behavioural Disorders, Duke-NUS Medical School, 8 College Road, Singapore169857, Singapore
3 Singapore-MIT Alliance for Research and Technology Centre, 1 Create Way, #10-01 Create Tower, Singapore 138602, Singapore
∥ X.B. and J.Y. contributed equally to this work.
10.1021/acs.analchem.0c02264
In-depth phenotyping of an HCC827 (lung adenocarcinoma) aggregoid (aggregated spheroid) 3D cell model by imaging mass cytometry. The overlay image shows the distribution of protein markers of epithelial tumor (E-cadherin (purple)), proliferation (Ki-67 (green)), hypoxia (Glut-1 (red)), and mitosis (pHH3 (white)). Data were acquired at 1 μm pixel size using the Hyperion Imaging System (Fluidigm) following staining of a 10 μm section with a cocktail of metal tagged antibodies.
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